Degradation of Fusobacterium nucleatum biofilm and quantity of reactive oxygen species due to a combination of photodynamic therapy and 2.5% sodium hypochlorite

Background: The persistence of microorganisms in the root canal system is one leading causes treatment failure. Biofilms putative pathogens hidden inside dentin tubules and other ramifications may limit current disinfection protocols. Photodynamic therapy (PDT) with a wavelength 628 nm can be used as an antimicrobial strategy that uses low-power laser energy to activate non-toxic photosensitizer produce singlet oxygen ability kill canals. Fusobacterium nucleatum was because this bacterium bacteria involved infection. Purpose: aim study compare bactericidal efficacy sodium hypochlorite (NaOCl) 2.5%, PDT, combination PDT NaOCl 2.5% against nucleatum. Methods: Mature biofilm divided into four groups according protocol decontamination: K1 (negative control – biofilm), K2 (NaOCl 2.5%), K3 (PDT), K4 + PDT). Biofilm degradation observed using optical density (OD) at 570 microplate reader. A reactive species quantity check carried out nitroblue tetrazolium test, OD observation done reader 540 nm. Results: Group 4 PDT) showed more elimination than groups. Conclusion: considered effective for There potentiation relationship between FotoSan. occurs effect antibacterial irradiation Toluidine blue O photosensitizer.